A Smart Circular Supply Chain (SCSC) integrates both Industry 4.0 (I4.0) and Circular Economy (CE) concepts into supply chain in response to achieving sustainable goals/agenda. The purpose of this paper is to assess SCSC readiness and maturity level of SMEs considering different stakeholders from a multi-layered perspective. For this aim, a conceptual framework was proposed and accomplished through a case study of SMEs in Turkey’s textile industry. Such integrated approach to holistically assessing SCSC readiness and maturity makes a unique contribution to the field. The highlights of this study are summarized as follows: (1) approaching readiness and maturity in transitions by focusing on systems theory; (2) identifying the dimensions of readiness and maturity in transitions to I4.0 and CE; (3) assessment of readiness and maturity level of SMEs in transition to CE and (4) assessment of readiness and maturity level of SMEs in transition to I4.0 within the supply chain.